| 高纯度大豆卵磷脂对中国仓鼠肺细胞染色体畸变作用 |
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| 引用本文： | 郭红刚,周莎桑,戴方伟,石巧娟,萨晓婴. 高纯度大豆卵磷脂对中国仓鼠肺细胞染色体畸变作用[J]. 中国实验动物学杂志, 2009, 0(8): 35-38,I0003 |
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| 作者姓名： | 郭红刚 周莎桑 戴方伟 石巧娟 萨晓婴 |
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| 作者单位： | 浙江省医学科学院,浙江省实验动物中心,浙江省实验动物与安全性研究重点实验室,杭州310013 |
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| 摘 要： | 目的研究高纯度大豆卵磷脂对中国仓鼠肺细胞（cHL）染色体畸变作用。方法测定高纯度大豆卵磷脂对CHL细胞的半数抑制浓度（IC50），根据IC50设立不同剂量组，进行正式试验，分别观察高纯度大豆卵磷脂接触CHL6h，24h及加S9后6h染色体的畸变情况，根据标准进行结果判定。结果染毒6h，24h及加S9后染毒6h染色体畸变为阴性。结论高纯度大豆卵磷脂不能引起CHL细胞染色体产生畸变作用。
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| 关 键 词： | 高纯度大豆卵磷脂 中国仓鼠肺细胞 染色体畸变 |
Highly Pure Lecithin Does not Affect Chromosome Aberration in Chinese Hamster Lung Cells |
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| GUO Hong-gang,ZHOU Sha-sang,DAI Fang-wei,SHI Qiao-juan,SA Xiao-ying. Highly Pure Lecithin Does not Affect Chromosome Aberration in Chinese Hamster Lung Cells[J]. Chinese Journal of Laboratory Animal Science, 2009, 0(8): 35-38,I0003 |
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| Authors: | GUO Hong-gang ZHOU Sha-sang DAI Fang-wei SHI Qiao-juan SA Xiao-ying |
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| Affiliation: | (Zhejiang Academy of Medical Sciences, Zhejiang Ccentre of Laboratory Animals, Zhejiang Provincial Laboratory of Experimental Animals ＆ non-Clinical Studies, Hangzhou 310013, China) |
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| Abstract: | Objective To explore if highly pure lecithin affect chromosome aberration in Chinese hamster lung （CHL） cells. Methods To determine the IC50 of highly pure lecithin on CHL cells, then to establish the range of doses according to the IC50 and to assess the cell chromosome aberration. The results were judged according to the positive response standard. Results Negative response was found at 6 h and 24 h after the treatment with highly pure lecithin and at 6 h after the addition of S9 mixture. Conclusions The highly pure lecithin does not induce chromosome aberration in CHL cells. |
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| Keywords: | Lecithin Chinese hamster lung cells （CHL） Chromosome aberration |
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