乳腺、甲状腺多原发癌患者乳腺癌组织中RETmRNA的表达及意义

目的：研究乳腺癌术后发生甲状腺癌患者乳腺癌组织中RET（rearranged during transfection）基因的表达及临床意义。方法：用逆转录-聚合酶链反应（RT-PCR）检测33例乳腺、甲状腺多原发癌患者乳腺癌组织中RET基因的酪氨酸激酶区（TK）的表达进行分析,并设30例单纯乳腺癌和20例乳腺良性病变做对照组。结果：（1）乳腺、甲状腺多原发癌患者乳腺癌组织中RET-TK阳性检测率达57.6%（19/33）,单纯乳腺癌患者癌组织中RET-TK阳性检出率30%（9/30）,乳腺良性病变患者病变乳腺组织中RET-TK阳性检出率为10%（2/20）。三组的阳性率差别分别都有统计学意义（P＆lt;0.05）。（2）RET-TK的阳性表达与患者的ER情况相关（P＆lt;0.05）,而与患者年龄、肿瘤直径、同侧腋窝淋巴结转移、Her-2表达等病理学特征无关（P＆gt;0.05）。结论：RET基因的过量表达可能与乳腺、甲状腺多原发癌的发生有关。

Expression of RETmRNA in malignant breast tissues of patients with multiple primary carcinomas in breast and thyroid and the clinical significance

Objective： To investigate the expression of RET （rearranged during transfection ）gene in malignant breast tissues of 33 patients who get thyroid cancer after malignant breast disease surgery and to investigate the clinicopathologic correlation. Methods： The expression of RET tyrosine kinase domain （RET-TK）of thirty-three specimens of malignant breast tissues from patients with multiple primary carcinomas in breast and thyroid were analyzed by RT-PCR, thirty specimens of malignant breast tissues of patients with breast cancer and twenty specimens of benign breast tissues were also included as control cases. Results： （ 1 ）57.6% of patients with multiple primary carcinomas in breast and thyroid （19/33） expressed RET tyrosine kinase domain （RET-TK）. 30% of patients with breast cancer （9/30） expressed RET-TK. 10%of patients with benign breast disease （2/20） expressed RET-TK. The positive rates of three groups were different significantly between any two groups respectively （P 〈0.05 ）. （2）Statistic analysis showed evident correlation be~＇een the expression of RET-TK and estrogen receptor （ER）（P〈0.05）, no significant difference was found regarding the age of the patients, tumor size, metastasis of lymph node and the expression of Her-2 state （P 〉0.05）. Conclusions： The activation of the RET gene is closely associated with the pathogenesis of multiple primary carcinomas in breast and thyroid.