不同方式构建A20鼠B细胞淋巴瘤动物模型

目的探索多种方式构建A20鼠B细胞淋巴瘤动物模型及不同方式造模成瘤的特征。方法鼠源性B细胞淋巴瘤细胞株A20经皮下、尾静脉、脾脏和腹腔接种于同源BALB/c小鼠或先接种裸鼠成瘤后组织块移植BALB/c小鼠,观察动物成瘤时间、成瘤率、成瘤部位;取肿瘤组织和动物脏器行石蜡包埋、病理切片、HE染色观察其组织学特点。结果BALB/c鼠皮下注2×10^6组、2×10^7组和裸鼠瘤组织移植BALB/c小鼠组成瘤率皆为100%,成瘤时间分别为（15.29±3.2）d（、7.0±0.82）d和（6.29±0.49）d。BALB/c小鼠尾静脉注射2×106组、2×107组、脾脏注射组、腹腔注射组成瘤率分别为71.4%、100%、71.4%、14.3%,成瘤时间分别为（76.8±12.0）d、（26.1±7.99）d、（32.6±5.99）d和27 d。尾静脉成瘤部位播及肝脏、脾脏、胰腺、肾脏、食道、胃、肠、肠系膜、脑、淋巴结、骨、子宫、肌肉等多脏器和组织。BALB/c鼠A20成瘤组织学类似人弥漫大B细胞淋巴瘤。结论成功构建A20皮下移植瘤模型、血行播散性模型,为利用有免疫功能动物进行B淋巴瘤相关研究提供了实验平台。

Construction of A20 Mouse B Lymphoma Animal Model in Different Ways

Objective To construct animal model using mouse B lymphoma A20 cell line in immunocompetent BALB/c mouse using different methods and compare the character/stics of tumor formation. Methods Inoculating mouse B lymphoma cell line A20 to homological BALB/c mouse by subcutaneous inoculation, intravenous injection, spleen inoculation, intraperitoneal injection and transplantation of tissue from nude mouse, observing the tumor formation, paraffin imbedding the organs of animals, pathological section and HE staining to observe its pathological characteristics. Results There were 100% tumor formation in the BALB/c mouse of subcutaneous inoculation （2 ×10^6and 2 ×10^7 ） groups and tissue （from nude mouse） trans-plantation group after inoculating about （ 15.29 ± 3.2） days, （7.0 ± 0.82） days and （6.29± 0.49） days, respectively. There were 71.4 % and 100 % tumor formation in intravenous injection （2 × 10^6 and 2× 10^7 ） group respectively, 71.4 % in spleen inoculation group and 14.3 % in intraperitoneal injection group after about （76.8 ± 12.0） days, （26.1 ± 7.99） days, （32.6 ± 5.99） days and 27 days. The tumor formed in several organs of mouse, such as liver, spleen pancreas, kidney, esophagus, stomach, intestine, mesentery, brain, lymphocytic node, bone, uterus and muscles et al. The pathological characteristics mimic human diffused large B cell lymphoma （DLBCL）. Conclusion The subcutaneously transplanted animal model and hematogenous disseminated animal models were successfully established, providing suitable animal models for research of B lymphomas in mouse with normal immune function.