大鼠脑缺血再灌注后组织蛋白酶D蛋白质及mRNA的表达

目的：探讨大鼠缺血再灌注后溶酶体组织蛋白酶D（Cathepsin DCD）在不同时段蛋白质及mRNA表达变化。方法：将60只SD大鼠随机分为三组：正常组（10只）,假手术组（10只）,脑缺血再灌注组（模型组）（40只）,线栓法制备大脑中动脉梗死模型（MCAO）,免疫组化及RT-PCR法分别检测CathepsinD的蛋白和mRNA表达。结果：与正常组和假手术组比较,模型组在大鼠脑缺血再灌注损伤后6hCathepsin D的蛋白和mRNA表达明显增强（P＆lt;0.05）,24h达高峰,48h仍保存高水平。结论：Cathepsin D在大鼠脑缺血再灌注后表达增强,溶酶体CathepsinD可能参与了脑缺血再灌注损伤后神经细胞凋亡。

Expression of the protein and mRNA of CathepsinD in Rats after Cerebral Ischemia-Reperfusion

Objective： To study the changes of the protein and mRNA of Cathepsin D expression in different time point in rat after cerebral ischemia-reperfusion. Methods： 60 SD rats were randomly divided into three groups： normal group （n=10）,sham-operation group （n=10）,cerebral ischemia-reperfusion group （n--40）, prepared by suture middle cerebral artery infarction model （MCAO）,the expression of the protein and mRNA of Cathepsin D were respectively evaluated by methods of immunohistochemistry and RT-PCR. Results： Compared with normal group and sham-operated group, The expression level of the protein and mRNA of Cathepsin D in the rats of model group increased significantly 6 hour and got to their peak 24 hour, kept a high level on 48h. Conclusions： The expression of Cathepsin D after ischemia-reperfusion is high, Cathepsin D may be involved in the process of neuronal apoptosis in the brain after ischemia-reperfusion injury.