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大鼠脑缺血再灌注后组织蛋白酶D蛋白质及mRNA的表达
引用本文:刘柳青,张智博,龚晓燕. 大鼠脑缺血再灌注后组织蛋白酶D蛋白质及mRNA的表达[J]. 生物磁学, 2009, 0(20): 3868-3870
作者姓名:刘柳青  张智博  龚晓燕
作者单位:[1]南华大学,湖南衡阳421001 [2]长沙市第一医院神经内科,湖南长沙410005
摘    要:目的:探讨大鼠缺血再灌注后溶酶体组织蛋白酶D(Cathepsin DCD)在不同时段蛋白质及mRNA表达变化。方法:将60只SD大鼠随机分为三组:正常组(10只),假手术组(10只),脑缺血再灌注组(模型组)(40只),线栓法制备大脑中动脉梗死模型(MCAO),免疫组化及RT-PCR法分别检测CathepsinD的蛋白和mRNA表达。结果:与正常组和假手术组比较,模型组在大鼠脑缺血再灌注损伤后6hCathepsin D的蛋白和mRNA表达明显增强(P<0.05),24h达高峰,48h仍保存高水平。结论:Cathepsin D在大鼠脑缺血再灌注后表达增强,溶酶体CathepsinD可能参与了脑缺血再灌注损伤后神经细胞凋亡。

关 键 词:脑缺血再灌注  溶酶体  Cathepsin  D

Expression of the protein and mRNA of CathepsinD in Rats after Cerebral Ischemia-Reperfusion
L,U Liu-qing,ZHANG Zhi-bo,GONG Xiao-yan. Expression of the protein and mRNA of CathepsinD in Rats after Cerebral Ischemia-Reperfusion[J]. Biomagnetism, 2009, 0(20): 3868-3870
Authors:L  U Liu-qing  ZHANG Zhi-bo  GONG Xiao-yan
Affiliation:1 Nanhua Universit, heng yang 421001, China; 2 Department of Neurology, The First Hospitol ofChangsha, Changsha 410005, China )
Abstract:Objective: To study the changes of the protein and mRNA of Cathepsin D expression in different time point in rat after cerebral ischemia-reperfusion. Methods: 60 SD rats were randomly divided into three groups: normal group (n=10),sham-operation group (n=10),cerebral ischemia-reperfusion group (n--40), prepared by suture middle cerebral artery infarction model (MCAO),the expression of the protein and mRNA of Cathepsin D were respectively evaluated by methods of immunohistochemistry and RT-PCR. Results: Compared with normal group and sham-operated group, The expression level of the protein and mRNA of Cathepsin D in the rats of model group increased significantly 6 hour and got to their peak 24 hour, kept a high level on 48h. Conclusions: The expression of Cathepsin D after ischemia-reperfusion is high, Cathepsin D may be involved in the process of neuronal apoptosis in the brain after ischemia-reperfusion injury.
Keywords:cerebral ischemia-reperfusion  lysosome  Cathepsin D
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