| 缺血再灌卒中后抑郁小鼠模型的建立 |
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| 引用本文： | 邢东明,国月英,炎彬,王道毅,王玉刚,杜力军. 缺血再灌卒中后抑郁小鼠模型的建立[J]. 中国实验动物学杂志, 2009, 0(3): 14-19 |
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| 作者姓名： | 邢东明 国月英 炎彬 王道毅 王玉刚 杜力军 |
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| 作者单位： | [1]清华大学生物科学与技术系药物药理研究室,北京100084 [2]中国中医科学院西苑医院,北京100083 |
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| 基金项目： | 本文受国家自然科学基金（30572340,90713043）和教育部博士学科点基金（20060003072,20070003039）资助. |
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| 摘 要： | 目的探讨并建立卒中后抑郁小鼠动物模型。方法先利用结扎颈总动脉造成小鼠不完性全脑缺血再灌注病理损伤，利用悬尾和强迫游泳方法诱导小鼠抑郁模型，利用正交试验设计全面考察其影响因素，确定最佳造模条件。结果以缺血5min，再灌10min，再缺血5min所诱导抑郁模型最为明显，且死亡率较低。结论利用此方法所造卒中后抑郁模型具有操作简便、成功率较高且经济的特点，适用于该类药物筛选。
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| 关 键 词： | 卒中后抑郁 小鼠 模型 |
Model Establishment of Post-stroke Depression in Mice |
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| XING Dong-ming,GUO Yue-ying,YAN Bin,WANG Dao-yi,WANG Yu-gang,DU Li-jun. Model Establishment of Post-stroke Depression in Mice[J]. Chinese Journal of Laboratory Animal Science, 2009, 0(3): 14-19 |
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| Authors: | XING Dong-ming GUO Yue-ying YAN Bin WANG Dao-yi WANG Yu-gang DU Li-jun |
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| Affiliation: | 1. Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China ; 2. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100083, China) |
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| Abstract: | Objective To study the model of post-stroke depression （PSD） with cerebral ischemia-reperfusion in mice. Methods First, the pathological changes of cerebral ischemia- reperfusion by ligaturing the cephalic arteries, then depression like behavior （immobility time） induced by tail suspension and forced swimming; the alteration analysis carried out by crossing test design. Results The best process of the model-induee is 5 minutes of blood isehemia, 10 minutes of blood reperfusion and 5 minutes of blood ischemia. Conclusion The model by the assay will be used for candidate drug screening in post stroke depression with simple operating, lower mortality and in economy. |
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| Keywords: | Post stroke depression Mouse Model |
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