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The archaeal exosome localizes to the membrane |
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| Authors: | Verena Roppelt Sonja V Albers Heinz Schwarz Elena Evguenieva-Hackenberg |
| Institution: | a Institut für Mikrobiologie und Molekularbiologie der JLU Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany b Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, D-72076 Tübingen, Germany c Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch Straße, D-35043 Marburg, Germany |
| Abstract: | We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.Structured summaryMINT-7891213: Rrp41 (uniprotkb:Q9UXC2) and DnaG (uniprotkb:P95980) colocalize (MI:0403) by cosedimentation in solution (MI:0028)MINT-7891235: Rrp41 (uniprotkb:Q9UXC2), DnaG (uniprotkb:P95980) and SlaA (uniprotkb:Q2M1E7) colocalize (MI:0403) by cosedimentation through density gradient (MI:0029)MINT-7891278: Rrp41 (uniprotkb:Q9UXC2) and DnaG (uniprotkb:P95980) colocalize (MI:0403) by fluorescence microscopy (MI:0416) |
| Keywords: | Archaea Localization Membrane Exosome Sulfolobus |
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