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Weird fingers: Functional analysis of WIP domain proteins |
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| Authors: | Ingo Appelhagen Gui-Hua Lu Bernd Weisshaar |
| Institution: | a Department of Biology, Chair of Genome Research, Bielefeld University, Universitätsstraße 27, D-33615 Bielefeld, Germany b Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Köln, Germany c Department of Genetics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Straße 24-25, D-14476 Potsdam-Golm, Germany |
| Abstract: | WIP proteins form a plant specific subfamily of C2H2 zinc finger (ZF) proteins. In this study, we functionally characterized the WIP domain, which consists of four ZF motifs, and discuss molecular functions for WIP proteins. Mutations in each of the ZFs lead to loss of function of the TT1/WIP1 protein in Arabiopsis thaliana. SV40 type nuclear localisation signals were detected in two of the ZFs and functionally characterized using GFP fusions as well as new mutant alleles identified by TILLING. Promoter swap experiments showed that selected WIP proteins are partially able to take over TT1 function. Activity of the AtBAN promoter, a potential TT1 target, could be increased by the addition of TT1 to the TT2-TT8-TTG1 regulatory complex. |
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