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Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats |
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| Authors: | Urs Duthaler Laura Rinaldi Mireille Vargas Jürg Utzinger Giuseppe Cringoli |
| Institution: | a Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland b University of Basel, P.O. Box, CH-4003 Basel, Switzerland c Department of Pathology and Animal Health, CREMOPAR Regione Campania, University of Naples ‘Federico II’, Via della Veterinaria 1, 80137 Naples, Italy d Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland |
| Abstract: | We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21 min to prepare and examine a single FLOTAC, whereas 114 min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P < 0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs. |
| Keywords: | Fasciola hepatica Diagnosis Sedimentation FLOTAC Sensitivity Faecal egg count Efficiency |
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