Overexpression of the single-stranded DNA-binding protein (SSB) stabilises CAG•CTG triplet repeats in an orientation dependent manner期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Overexpression of the single-stranded DNA-binding protein (SSB) stabilises CAG•CTG triplet repeats in an orientation dependent manner

Authors:	Federica Andreoni Wilson C.K. Poon

Affiliation:	^a Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Darwin Building, King’s Buildings, Edinburgh, EH9 3JR, UK ^b SUPA and School of Physics, University of Edinburgh, James Clerk Maxwell Building, King’s Buildings, Mayfield Road, Edinburgh EH9 3JZ, UK

Abstract:	The stability and deletion-size-distribution profiles of leading strand (CAG)₇₅ and (CTG)₁₃₇ trinucleotide repeat arrays inserted in the Escherichia coli chromosome were investigated upon overexpression of the single-stranded DNA-binding protein (SSB) and in mutant strains deficient for the SbcCD (Rad51/Mre11) nuclease. SSB overexpression increases the stability of the (CAG)₇₅ repeat array and leads to a loss of the bias towards large deletions for the same array. Furthermore, the absence of SbcCD leads to a reduction in the number of large deletions in strains containing the (CTG)₁₃₇ repeat array.

Keywords:	TNR, trinucleotide repeat SSB, single-stranded DNA-binding protein amp, ampicillin ssDNA, single-stranded DNA
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