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Taenia saginata: Production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens |
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| Authors: | Josy Campanhã Vicentini-Oliveira,Silvana de Cá ssia Paulan,Rosana Rossi-Ferreira,Cá ris Maroni Nunes |
| Affiliation: | a UNESP, Univ. Estadual Paulista, Campus Botucatu, Graduation Program on Veterinary Medicine, School of Veterinary Medicine and Zootechny, 18618-000 Botucatu, SP, Brazil b UNESP, Univ. Estadual Paulista, Campus Botucatu, Flowcitometry Laboratory, Blood Center, Faculty of Medicine, 18618-970 Botucatu, SP, Brazil c UNESP, Univ. Estadual Paulista, Campus Araçatuba, Graduation Program on Animal Science, 16050-680 Araçatuba, SP, Brazil d UNESP, Univ. Estadual Paulista, Campus Botucatu, Department of Hygiene and Public Health, School of Veterinary Medicine and Zootechny, 18618-000 Botucatu, SP, Brazil e UNESP, Univ. Estadual Paulista, Campus Bauru, Department of Biological Sciences, Faculty of Science, 17033-360 Bauru, SP, Brazil f UNESP, Univ. Estadual Paulista, Campus Botucatu, Cellular Engineering Laboratory, Blood Center, Faculty of Medicine, 18618-970 Botucatu, SP, Brazil g UNESP, Univ. Estadual Paulista, Department of Animal Health and Production, Rua Clóvis Pestana, 793, Jd. D. Amélia, 16050-680 Araçatuba, SP, Brazil |
| Abstract: | Cysticercosis is a major cause of economic loss in bovine production due to meat condemnation. Chemotherapy is being used in Brazilian cattle and a diagnostic test to improve the treatment program is desired. We produced monoclonal antibodies against crude (TAEB) and cyst fluid (TAEF) Taenia saginata metacestode antigens using immunized BALB/c mice. After cell fusion, 10 TAEB and nine TAEF hybrids were selected and cloned resulting in 18 IgG1 and 32 IgM TAEB clones, and 9 IgG1 and 9 IgM TAEF clones. Ascites was produced and Western blot testing was performed resulting in reactivity to protein fractions of low molecular weight (<18 kDa), 43, 55, 66 and 100 kDa. The indirect immunofluorescence test, with one monoclonal antibody against crude and one against cyst fluid antigens, recognized antigenic fractions of both the scolex and the bladder wall of metacestodes from naturally infected bovine. |
| Keywords: | Monoclonal antibody (MAb) Taenia saginata Bovine cysticercosis Crude antigen Cyst fluid |
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