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Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly |
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| Authors: | Aaron G Smith Carol B Johnson Stanislav Vitha |
| Institution: | a Microscopy and Imaging Center, Texas A&M University, College Station, TX 77843-2257, USA b Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA c Department of Biology, Texas A&M University, College Station, TX 77843, USA |
| Abstract: | Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II filaments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca2+. |
| Keywords: | FtsZ filamentous temperature sensitive Z ARC accumulation and replication of chloroplasts FtsZ1 FtsZ1-1 from Arabidopsis thaliana FtsZ2 FtsZ2-1 from Arabidopsis thaliana |
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