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Efficacy of S-adenosylhomocysteine hydrolase inhibitors, D-eritadenine and (S)-DHPA, against the growth of Cryptosporidium parvum in vitro |
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| Authors: | Vlasta ?trná ctá ,Jason M. Fritzler,Má ria &Scaron urinová ,Guan Zhu |
| Affiliation: | a Charles University in Prague and University Hospital Bulovka, First Faculty of Medicine, Department of Infectious and Tropical Diseases, Prague, Czech Republic b Academy of Sciences of the Czech Republic, Institute of Biotechnology, Prague, Czech Republic c Stephen F. Austin State University, College of Sciences and Mathematics, Department of Biology, Nacogdoches, TX 75962, USA d Charles University in Prague, Faculty of Sciences, Department of Parasitology, Prague, Czech Republic e Texas A&M University, College of Veterinary Medicine and Biomedical Sciences, Department of Veterinary Pathobiology, College Station, TX 77843-4467, USA |
| Abstract: | D-eritadenine and (S)-DHPA are aliphatic adenosine analogues known to target S-adenosylhomocysteine hydrolase (SAHH) and potent antiviral compounds. In the present study, we demonstrate that these two compounds also display efficacy against recombinant SAHH enzyme of the protozoan parasite Cryptosporidium parvum, as well as inhibition of parasite growth in vitro. Our data confirm that SAHH could serve as a rational drug target in cryptosporidial infection and antiviral adenosine analogues are potential candidates for drug development against cryptosporidiosis. |
| Keywords: | Cryptosporidium parvum Apicomplexa S-adenosylhomocysteine hydrolase E.C. 3.3.1.1 D-eritadenine (S)-DHPA Paromomycin RT-qPCR |
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