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Tudor-SN interacts with and co-localizes with G3BP in stress granules under stress conditions |
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| Authors: | Xingjie Gao Lin Ge Jie Shao Chao Su Hong Zhao Juha Saarikettu Zhi Yao Olli Silvennoinen Jie Yang |
| Institution: | a Department of Immunology, Basic Medical College, Tianjin Medical University, PR China b Tianjin Key Laboratory of Cellular and Molecular Immunology, PR China c Key Laboratory of Educational Ministry of China, Heping District Qixiangtai Road No. 22, Tianjin 300070, PR China d Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, PR China e Institute of Medical Technology, University of Tampere, Biokatu 8, FI-33014 Tampere, Finland f Department of Clinical Microbiology, Tampere University Hospital, FI-33520 Tampere, Finland |
| Abstract: | SGs are mRNA containing cytoplasmic structures that are assembled in response to stress. Tudor-SN protein is a ubiquitously expressed protein. Here, Tudor-SN protein was found to physiologically interact with G3BP, which is the marker and effector of SG. The kinetics of the assembly of SGs in the living cells demonstrated that Tudor-SN co-localizes with G3BP and is recruited to the same SGs in response to different stress stimuli. Knockdown of endogenous Tudor-SN did not inhibit the formation of SGs, but retarded the aggregation of small SGs into large SGs. Thus Tudor-SN may not be an initiator as essential as G3BP for the formation of SGs, but affects the aggregation of SGs. These findings identify Tudor-SN as a novel component of SGs.Structured summaryMINT-7968768, MINT-7968779: Tudor-SN (uniprotkb:Q7KZF4) physically interacts (MI:0915) with G3BP (uniprotkb:Q13283) by anti bait coimmunoprecipitation (MI:0006) MINT-7968800: Tudor-SN (uniprotkb:Q7KZF4) and TIA-1 (uniprotkb:P31483) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7968789: Tudor-SN (uniprotkb:Q7KZF4) and G3BP (uniprotkb:Q13283) colocalize (MI:0403) by fluorescence microscopy (MI:0416) |
| Keywords: | Tudor-SN G3BP Stress granule Tudor domain containing protein |
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