|
|||||
|
|
Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts |
|
| Authors: | Tsuyoshi Sato Takahiro Abe Norimichi Nakamoto Shoichiro Kokabu Yasuhisa Tomaru Michihiko Usui Tetsuya Yoda |
| Institution: | a Department of Oral and Maxillofacial Surgery, Saitama Medical University, 38 Moro-hongou, Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan b Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan c Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjyuku-ku, Tokyo 162-8666, Japan d Department of Periodontology, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan e Department of Oral Surgery, Gunma Prefectural Cancer Center, 617-1 Takabayashi-nishi-machi, Ohta-shi, Gunma 373-8550, Japan |
| Abstract: | Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts. |
| Keywords: | Acetylcholine Cholinergic receptor Cholinergic component Osteoblast Proliferation Differentiation |
| 本文献已被 ScienceDirect 等数据库收录！ | |