N-Glycosylation patterns of hemolymph glycoproteins from Biomphalaria glabrata strains expressing different susceptibility to Schistosoma mansoni infection |
| |
Authors: | Tobias Lehr Sandra Frank Shunji Natsuka Hildegard Geyer Michael J Doenhoff Rudolf Geyer |
| |
Institution: | a Institute of Biochemistry, Faculty of Medicine, University of Giessen, D-35392 Giessen, Germany b Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan c Rudolf-Buchheim-Institute of Pharmacology, Faculty of Medicine, University of Giessen, D-35392 Giessen, Germany d School of Biology, University of Nottingham, Nottingham NG7 2RD, UK |
| |
Abstract: | Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (β1-2)-linked xylose or terminal Fuc(α1-3)GalNAc(β1-4)±Fuc(α1-3)]GlcNAc(β1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host. |
| |
Keywords: | Trematode Schistosoma mansoni infection Susceptible snails Resistant snails Molecular mimicry Glycoprotein Carbohydrate epitope Oligosaccharide mapping Mass spectrometry |
本文献已被 ScienceDirect 等数据库收录! |
|