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Taenia crassiceps cysticerci: Characterization of the 14-kDa glycoprotein with homologies to antigens from Taenia solium cysticerci |
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| Authors: | Regina H Peralta Alessandra X Pardini Hercules Moura Adelaide J Vaz |
| Institution: | a Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil b Laboratório de Imunologia Clínica, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil c National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, USA d Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil |
| Abstract: | Glycoproteins from the total vesicular fluid of Taenia crassiceps (VF-Tc) were prepared using three different purification methods, consisting of ConA-lectin affinity chromatography (ConA-Tc), preparative electrophoresis (SDS-PAGE) (14gp-Tc), and monoclonal antibody immunoaffinity chromatography (18/14-Tc). The complex composition represented by the VF-Tc and ConA-Tc antigens revealed peptides ranging from 101- to 14-kDa and from 92- to 12-kDa, respectively. Immunoblotting using lectins confirmed glucose/mannose (glc/man) residues in the 18- and 14-kDa peptides, which are considered specific and immunodominant for the diagnosis of cysticercosis, and indicated that these fractions are glycoproteins. Serum antibodies from a patient with neurocysticercosis that reacted to the 14gp band from T. crassiceps (Tc) were eluted from immunoblotting membranes and showed reactivity to 14gp from Taenia solium. In order to determine the similar peptide sequence, the N-terminal amino acid was determined and analyzed with sequences available in public databases. This sequence revealed partial homology between T. crassiceps and T. solium peptides. In addition, mass spectrometry along with theoretical Mr and pI of the 14gp-Tc point suggested a close relationship to some peptides of a 150-kDa protein complex of the T. solium previously described. The identification of these common immunogenic sites will contribute to future efforts to develop recombinant antigens and synthetic peptides for immunological assays. |
| Keywords: | NC neurocysticercosis CSF cerebro spinal fluid Tc T crassiceps Tso T solium VF-Tc vesicular fluid from Tc antigen ConA-Tc fractions of VF-Tc purified by Concanavalin A 14gp-Tc 14gp purified by preparative SDS-PAGE 18/14-Tc 18- and 14-kDa proteins from VF-Tc obtained by immunoaffinity chromatography |
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