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Inflammatory changes in the central nervous system are associated with behavioral impairment in Plasmodium berghei (strain ANKA)-infected mice |
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| Authors: | Norinne Lacerda-Queiroz,David Henrique Rodrigues,Má rcia Carvalho Vilela,Dé bora Cristina Guerra Amaral,Leonardo José de Moura Carvalho,Charles L Howe,Antô nio Lú cio Teixeira |
| Affiliation: | a Department of Cellular Biology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil b Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, UFMG, Belo Horizonte, Brazil c Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil d La Jolla Bioengineering Institute, La Jolla, CA, USA e Departments of Neurology and Neuroscience, Mayo Clinic College of Medicine, Rochester, MN, USA f Neurology Group, Department of Internal Medicine, School of Medicine, UFMG, Belo Horizonte, Brazil |
| Abstract: | Experimental cerebral malaria is a neuroinflammatory condition that results from the host immune response to the parasite. Using intravital microscopy, we investigated leukocyte recruitment in the brain microcirculation and the temporal relationship of this process to the behavioral changes observed in Plasmodium berghei (strain ANKA)-infected C57Bl/6 mice. We found that leukocyte recruitment was increased from day 5 post-infection (p.i.) onwards. Histopathological changes and increased levels of inflammatory cytokines in the brain were also observed. Behavioral performance evaluated by the SHIRPA protocol showed functional impairment from day 6 p.i. onwards. Thus, early leukocyte migration into the brain and associated inflammatory changes may be involved in neurological impairment in parasite-infected C57Bl/6 mice. |
| Keywords: | Cerebral malaria Leukocyte recruitment Behavior Cytokines Intravital microscopy |
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