NSF independent fusion of Salmonella-containing late phagosomes with early endosomes |
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Authors: | Seetharaman Parashuraman Amitabha Mukhopadhyay |
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Affiliation: | National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India |
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Abstract: | Initial characterizations of live-Salmonella-containing early (LSEP) and late phagosomes (LSLP) in macrophages show that both phagosomes retain Rab5 and EEA1. In addition, LSEP specifically contain transferrin receptor whereas LSLP possess relatively more rabaptin-5. In contrast to LSLP, late-Salmonella-containing vacuoles in epithelial cells show significantly reduced levels of Rab5 and EEA1. Subsequent results demonstrate that both phagosomes efficiently fuse with early endosomes (EE). In contrast to LSEP, fusion between LSLP and EE is insensitive to ATPγS treatment. Furthermore, LSLP fuses with EE in absence of NEM-sensitive fusion factor (NSF) as well as in the presence of NSF:D1EQ mutant demonstrating that LSLP fusion with EE is NSF independent. |
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Keywords: | LSEP, live Salmonella-containing early phagosomes LSLP, live Salmonella-containing late phagosomes NEM, N-ethylmaleimide NSF, NEM-sensitive fusion factor HRP, horse radish peroxidase IM, internalization medium HB, homogenization buffer PNS, post-nuclear supernatant |
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