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Plasmodium vivax: Comparison of the immune responses between oral and parenteral immunization of rPv54 in BALB/c mice |
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| Authors: | Myoung-Hee Kwon Hyung-Hwan Kim Ho-Sa Lee Tong-Soo Kim Yong-Joo Ahn Youngjoo Sohn Hyeong-Woo Lee |
| Affiliation: | a Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea b Vascular Medicine Research Unit, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA 02139, USA c Institute of Global Environment and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea d Department of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea e Department of Gynecology, College of Oriental Medicine, Sangji University, Wonju 220-717, Republic of Korea f International Research Center for Biology and Biotechnology, Goesan 367-805, Republic of Korea g Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, FL 32610, USA |
| Abstract: | The merozoite surface protein-1 (MSP-1) from Plasmodium vivax was evaluated as an oral vaccine candidate by cloning and expressing the interspecies conserved block 10 (ICB10) of the MSP-1 from a Korean isolate in Escherichia coli. The expressed fusion protein contained ICB10 and a maltose-binding protein (MBP), rPv54, has a molecular weight of approximately 54 kDa as determined by SDS-PAGE analysis. IgG against rPv54 was successfully produced in BALB/c mice by oral immunization and sustained for more than 4 months. IgG2b was dominantly produced in both oral and parenteral immunizations. The rPv54 increased the frequency of NK, NKT, CD4+ T, CD8+ T, and B cells in both immunizations. IL-5 and TNF-α were increased in both significantly. In conclusion, rPv54 might be a valuable potential vaccine candidate for the oral and parenteral immunization against vivax malaria. |
| Keywords: | Merozoite surface protein-1 Plasmodium vivax Oral immunization ICB10 |
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