Biochemical characterisation of Murray Valley encephalitis virus proteinase |
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Authors: | Joma Joy |
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Affiliation: | Experimental Therapeutics Centre, Agency for Science, Technology and Research (A∗Star), 31 Biopolis Way, #03-01 Nanos, Singapore 138669, Singapore |
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Abstract: | Murray Valley encephalitis virus (MVEV) is a member of the flavivirus group, a large family of single stranded RNA viruses, which cause serious disease in all regions of the world. Its genome encodes a large polyprotein which is processed by both host proteinases and a virally encoded serine proteinase, non-structural protein 3 (NS3). NS3, an essential viral enzyme, requires another virally encoded protein cofactor, NS2B, for proteolytic activity. The cloning, expression and biochemical characterisation of a stable MVEV NS2B-NS3 fusion protein is described. |
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Keywords: | Flavivirus Protease Inhibitor Murray Valley encephalitis Dengue West Nile virus |
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