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Neospora caninum: Early immune response of rat mixed glial cultures after tachyzoites infection |
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| Authors: | AM Pinheiro SL Costa CSO Ribeiro RS El-Bachá |
| Institution: | a Laboratório de Neuroquímica e Biologia Celular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Brazil b Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Brazil c Laboratory of Cell Plasticity and Therapy Inserm (U-421) and UPVM Paris XII, France d Laboratório de Bioquímica e Imunologia Veterinária, Centro de Ciências Agrárias, Ambientais e Biológicas, Campus da Universidade Federal do Recôncavo da Bahia, 44.380-000, Cruz das Almas - BA, Brazil |
| Abstract: | Neospora caninum causes neurologic disease in dogs and abortion in cattle. Little is known about the immune response of the CNS against this protozoan. The aim of this study was to evaluate production of IL-6, IL-10, TNF-α, IFN-γ, and NO in rat mixed glial cell cultures infected by N. caninum. IFN-γ was not observed. The mean cytokine released after 24 and 72 h of infection were 3.8 ± 0.6 and 3.7 ± 0.6 pg TNF-α/mg protein and 2.7 ± 0.69 and 4.1 ± 0.64 pg IL-10/mg protein, respectively, and more than 8.0 pg IL-6/mg protein for both time points. NO levels increased 24 h post-infection (2.3 ± 0.8 pg/mg protein) until 72 h (4.2 ± 1.1 pg/mg protein) and the number of tachyzoites reduced with the time. Our results show high levels of regulatory cytokines that may suppress the harmful effects of IFN-γ; high levels of TNF-α and NO may represent an effective response by infected glial cells against N. caninum. |
| Keywords: | Neospora caninum Immune response CNS Cytokines |
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