Yeast acetic acid-induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1 |
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| Authors: | Nicoletta Guaragnella Antonella Bobba Ersilia Marra Sergio Giannattasio |
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| Affiliation: | a CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, Italy
b Dipartimento di Scienze per la Salute, Università del Molise, I-86100 Campobasso, Italy |
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| Abstract: | To investigate the role of cytochrome c (cyt c) release in yeast acetic acid-induced programmed cell death (AA-PCD), wild type (wt) and cells lacking metacaspase (Δyca1), cytochrome c (Δcyc1,7) and both (Δcyc1,7Δyca1) were compared for AA-PCD occurrence, hydrogen peroxide (H2O2) production and caspase activity. AA-PCD occurs in Δcyc1,7 and Δcyc1,7Δyca1 cells slower than in wt, but similar to that in Δyca1 cells, in which no cytochrome c release occurs. Both H2O2 production and caspase activation occur in these cells with early and extra-activation in Δcyc1,7 cells. We conclude that alternative death pathways can be activated in yeast AA-PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it. |
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| Keywords: | AA, acetic acid PCD, programmed cell death AA-PCD, acetic acid-induced programmed cell death wt, wild type Δyca1, YCA1-lacking Δcyc1,7, cytochrome c lacking H2O2, hydrogen peroxide cyt c, cytochrome c DCF, dichlorofluorescein diacetate z-VAD-fmk, carbobenzoxy-valyl-alanyl-aspartyl-fluoromethylketone |
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