PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction |
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| Authors: | Saiwasan Buathong Peerapan Tan-ariya Paanjit Taamasri Saovanee Leelayoova |
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| Affiliation: | a Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
b Department of Parasitology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand |
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| Abstract: | This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4 °C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious. |
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| Keywords: | Opisthorchis PCR DNA extraction Miracidia PBS incubation |
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