High-resolution mapping of the <Emphasis Type="Italic">Alp</Emphasis> locus and identification of a candidate gene <Emphasis Type="Italic">HvMATE</Emphasis> controlling aluminium tolerance in barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) |
| |
Authors: | Junping Wang Harsh Raman Meixue Zhou Peter R Ryan Emmanuel Delhaize Diane M Hebb Neil Coombes Neville Mendham |
| |
Institution: | (1) Tasmanian Institute of Agricultural Research and School of Agricultural Science, University of Tasmania, P.O. Box 46, Kings Meadows, TAS, 6249, Australia;(2) NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, PMB, Wagga Wagga, NSW, 2650, Australia;(3) CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia;(4) Tasmanian Institute of Agricultural Research and School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia |
| |
Abstract: | Aluminium (Al) tolerance in barley is conditioned by the Alp locus on the long arm of chromosome 4H, which is associated with Al-activated release of citrate from roots. We developed
a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070
F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated efflux of citrate from the root apices
of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715,
Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with the Alp locus in the DH population accounting 72% of the variation for Al tolerance evaluated as relative root elongation. These
markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and
ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not polymorphic in the Dayton/Gairdner population we instead investigated
the expression of the HvMATE gene. Relative expression of the HvMATE gene was 30-fold greater in Dayton than Gardiner. Furthermore, HvMATE expression in the F2:3 families tested, including all the informative recombinant lines identified between HvGABP and ABG715 was significantly correlated
with Al tolerance and Al-activated citrate efflux. These results identify HvMATE, a gene encoding a multidrug and toxic compound extrusion protein, as a candidate controlling Al tolerance in barley. |
| |
Keywords: | Aluminium tolerance Barley Citrate efflux Comparative mapping MATE Molecular markers |
本文献已被 PubMed SpringerLink 等数据库收录! |
|