Changes in optical path difference in the oesophageal region and the excretory cells during exsheathment in Haemonchus contortus |
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Authors: | KG Davey RI Sommerville |
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Institution: | Department of Zoology, University of Adelaide, Adelaide, S.A., Australia |
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Abstract: | Davey K. G. and Sommerville R. I. 1982. Changes in optical path difference in the oesophageal region and the excretory cells during exsheathment in Haemonchus contortus. International Journal for Parasitology12: 503–507. Changes in the optical path difference (opd) between various parts of the worm and the medium in which the worms were immersed were determined by quantitative interference microscopy. The opd of the oesophagus and the excretory cells both increased upon stimulation of the worms with CO2 at 38.5°C, suggesting a decrease in volume of those structures. The oesophagus decreased markedly in length and slightly in diameter, yielding a decrease in volume of approximately 15 to 17 pl. Desheathing the worms with NaOCl produced changes in the oesophagus but not the excretory cells. This confirms previous findings that exsheathment involves at least two parallel processes, both of which are initiated by CO2 and only one of which is stimulated by exposure to NaOCl. |
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Keywords: | Nematode development water content interference microscopy |
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