The Suppressor of fused gene, involved in Hedgehog signal transduction in Drosophila, is conserved in mammals |
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Authors: | M Delattre Sandrine Briand Mélanie Paces-Fessy Marie-Françoise Blanchet-Tournier |
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Institution: | (1) Institut Jacques Monod, Laboratoire ”Génétique du Développement et Evolution”, Universités Pierre et Marie Curie et Denis Diderot, 2 place Jussieu, F-75251 Paris cedex 05, France, FR |
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Abstract: | The Suppressor of fused Su(fu)] gene of Drosophila melanogaster encodes a protein containing a PEST sequence sequence enriched in proline (P), glutamic acid (E), serine (S) and threonine
(T)] which acts as an antagonist to the serine-threonine kinase Fused in Hedgehog (Hh) signal transduction during embryogenesis.
The Su(fu) gene isolated from a distantly related Drosophila species, D. virilis, shows significantly high homology throughout its protein sequence with its D. melanogaster counterpart. We show that these two Drosophila homologs of Su(fu) are functionally interchangeable in enhancing the fused phenotype. We have also isolated mammalian homologs of Su(fu). The absence of the PEST sequence in the mammalian Su(fu) protein suggests a different regulation for this product between
fly and vertebrates. Using the yeast two-hybrid method, we show that the murine Su(fu) protein can interact directly with
the Fused and Cubitus interruptus proteins, known partners of Su(fu) in Drosophila. These data are discussed in the light of their evolutionary relationships.
Received: 11 September 1998 / Accepted: 9 December 1998 |
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Keywords: | Evolution Suppressor of fused Hedgehog Development |
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