Twelve novel polymorphic microsatellite loci developed from the Asiatic black bear (<Emphasis Type="Italic">Ursus thibetanus</Emphasis>) |
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Authors: | Hua Wu Shan-Ning Zhang Fu-Wen Wei |
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Institution: | (1) Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichengxilu, Chaoyang District, 100101 Beijing, China;(2) Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079 Wuhan, China;(3) China Wildlife Conservation Association, 100714 Beijing, China; |
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Abstract: | Since the number and range of Asiatic black bear (Ursus thibetanus) are declining due to habitat loss and illegal trade, it is essential to take effective actions to reinforce the conservation
of the remaining bear populations. In order to aid such conservation efforts, we developed 12 novel polymorphic microsatellite
loci of Asiatic black bear from genomic DNA-enriched libraries in this paper. The number of alleles per locus in 24 individuals
ranged from 3 to 10, the average observed heterozygosity per locus from 0.214 to 0.950, and the average expected heterozygosity
per locus from 0.243 to 0.891. Eight loci followed Hardy–Weinberg expectations after Bonferroni correction for multiple comparisons.
No significant linkage association was found among all these loci. The 12 polymorphic microsatellite loci will be helpful
to the conservation of the Asiatic black bear. |
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Keywords: | |
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