Genomic organization and analysis of the 5' end of the porcine ryanodine receptor gene (ryr1). |
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| Authors: | B Brenig G Brem |
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| Institution: | Lehrstuhl für Molekulare Tierzucht, Veterin?rmedizinische Fakult?t Ludwig-Maximilians-Universit?t München, Germany. |
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| Abstract: | In this study we describe the isolation of genomic clones of the 5' region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs. |
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