蔗糖浓度对三裂叶野葛毛状根生长及其次生物质产生的影响

本文研究了蔗糖浓度对发根农杆菌ATCC15834诱导产生的三裂叶野葛毛状根生长及其葛根素和异黄酮类化合物产生的影响以及液体培养基中蔗糖的消耗变化。结果表明：毛状根在含5％、4％、3％和2％蔗糖的MS培养基中培养16天后的干重增殖倍数分别为11．7、11．9、10．1和5．9；其中尤以3％的蔗糖浓度最有利于毛状根中异黄酮类化合物及葛根素的积累；培养12天后，毛状根的葛根素含量达到最高，约5．147mg／gDW；而其异黄酮类化合物的含量则在培养16天后达到最高，约27．76mg／gDW。在毛状根液体培养过程中培养基的蔗糖浓度随着毛状根的生长而降低，其消耗速率与毛状根的生长速度及其可溶性总糖含量成正比。毛状根的可溶性总糖含量在培养12天时达到最高，而培养16天后培养基中的蔗糖消耗完毕。

Effect of sucrose concentration on the growth and production of secondary metabolites in Pueraria phaseoloides hairy roots]

Effects of sucrose concentrations on the growth and production of puerarin and isoflavones compounds in Pueraria phaseoloides hairy roots induced by Agrobacterium rhizogenes ATCC15834 were investigated. Changes of sucrose consumption in the medium during liquid culture were also determined. The results showed cultured for 16 days in MS medium with 5%, 4%, 3% and 2% sucrose, the proliferation times of dry weight of hairy roots were 11.7, 11.9, 10.1 and 5.9, respectively. 3% sucrose concentration in liquid medium was the best for accumulation of puerarin and isoflavones in the hairy roots. The highest content of puerarin, 5.147 mg/g DW, was obtained after 12 days of liquid culturing while the highest content of isoflavones, about 27.76 mg.g.DW, was gained after 16 days in culturing. Sucrose concentration decreased as hairy root growth proceeded. The growth rate and the content of soluble sugar in hairy roots of P. phaseoloides was directly proportional to the rate of sucrose utilization in the liquid medium during the whole culture. It was observed that the highest content of soluble sugar in hairy roots was at day 12 of liquid culture and sucrose in the liquid medium was used up at the end of 16 days of culture.