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大铃棉中棉所48主要经济性状的QTL定位分析
引用本文:潘兆娥,贾银华,孙君灵,周忠丽,何守朴,王立如,庞保印,杜雄明. 大铃棉中棉所48主要经济性状的QTL定位分析[J]. 植物遗传资源学报, 2011, 12(4)
作者姓名:潘兆娥  贾银华  孙君灵  周忠丽  何守朴  王立如  庞保印  杜雄明
作者单位:中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳,455000
基金项目:国家“863”计划项目
摘    要:利用4961对SSR引物对中棉所48的2个亲本进行多态性筛选,结果筛选到71对条带清晰、稳定性好的多态性引物,利用这些引物对261个F2群体进行扩增构建连锁图,获得了包含有49个标记位点的14个连锁群,共覆盖498.7cM,约占棉花总基因组10.0%的遗传图谱.采用WinQTL Cartographer 2.5的复合区间作图法(CIM),对F2群体的铃重、衣分及纤维品质进行分析,共检测到l1个稳定的QTLs,其中铃重2个、表分4个、纤维整齐度2个、纤维细度2个、纤维伸长率1个.这些稳定表达的QTLs能解释较大的表型变异,可用于纤维品质及产量性状的标记辅助选择,也可为大铃、优质棉的分子标记辅助选择,改良、提高大铃基因的选择效率,提供理论依据.

关 键 词:大铃棉  中棉所48  分子标记  SSR  QTLs

QTLs Location of Economic Traits for CCRI 48 with Big Bolls
PAN Zhao-e,JIA Yin-hua,SUN Jun-ling,ZHOU Zhong-li,HE Shou-pu,WANG Li-ru,PANG Bao-yin,DU Xiong-ming. QTLs Location of Economic Traits for CCRI 48 with Big Bolls[J]. Journal of Plant Genetic Resources, 2011, 12(4)
Authors:PAN Zhao-e  JIA Yin-hua  SUN Jun-ling  ZHOU Zhong-li  HE Shou-pu  WANG Li-ru  PANG Bao-yin  DU Xiong-ming
Affiliation:PAN Zhao-e,JIA Yin-hua,SUN Jun-ling,ZHOU Zhong-li,HE Shou-pu,WANG Li-ru,PANG Bao-yin,DU Xiong-ming(Key Laboratory of Cotton Genetic Improvement,the Ministry of Agriculture/Cotton ResearchInstitute of Chinese Academy of Agricultural Science,Anyang Henan 455000)
Abstract:4961 pairs of different sources SSR primers were used to screen polymorphism between two parents.71 pairs of them with stable amplification and clear bands were used to amplify among the 261 F2 and resulted into 71 polymorphism loci.Using the Joinmap 3.0 software with LOD of 3.0,49 loci was grouped into 14 linkage groups and covered 498.7cM,approximately 10.0% of the total recombinational length of the cotton genome.13 groups had been anchored to the chromosomes.A total of 11 stable QTLs,2 for boll weight,4...
Keywords:Big boll cotton  CCRI 48  Molecular marker  SSR  QTLs  
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