利用RIL和CSSL群体检测水稻种子休眠性QTL

利用由梗稻品种Asominori与籼稻品种IR24的杂交组合衍生的重组自交F10。家系(Recombinant Inbred Lines,RIL)群体及其衍生的染色体片段置换系(Chromosome Segment Substitution Lines,CSSL)群体，进行了种子休眠性QTL的检测和遗传效应分析。其中CSSL群体有2个，即CSSLl(以Asominori为背景，置换片段来自IR24)和CSSL2(以IR24为背景，置换片段来自Asominori)。在RIL群体上共检测到3个种子休眠性QTL，分别位于第3、6和9染色体上；在CSSL1群体中检测到分布在第1、3和7染色体上的3个休眠性QTL；而在CSSl2群体上检测到的3个QTL则分别位于第1、2和7染色体上。同时在两套CSSL群体上，分别检测到位于第1、7染色体上位置相近且效应一致的休眠性QTL，分析表明其所在的Asominori片段含对种子休眠性的增效基因，相应的IB24段含有减效基因。

Detection and Analysis of QTL for Seed Dormancy in Rice (Oryza sativa L.) Using RIL and CSSL Population

A recombinant inbred line (RIL) population and two chromosome segment substitution line (CSSL) population derived from the cross of Asominori (japonica) and IR24 (indica) were used to detect QTL controlling seed dormancy.CSSL1 were a series of IR24 chromosome segment substitution lines in Asominori background,and CSSL2 were a series of introgression lines of Asominori in the background of IR24.Three QTL were detected on chromosome 3,6 and 9 in RIL population,and individual QTL accounted for between 12.3% and 13%.Three QTL were detected on chromosome 1,3 and 7 in CSSL1,and individual QTL accounted for between 11.5% and 18.9%.Three QTL were detected on chromosome 1,2 and 7 in CSSL2,and individual QTL accounted for between 11% and 16%.The QTLs on chromosome 1 and 7 were detected in CSSL1 and CSSL2 populations simultaneously,QTL came from Asomonori, the moderate dormant cultivar,increased seed dormancy,and QTL from IR24,the weakly dormant cultivar,decreased seed dormancy.It can be deduced that there exist genes controlling seed dormancy at this region.