A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology |
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| Authors: | Christopher N Miller Lyne Jossé Ian Brown Ben Blakeman Jane Povey Lyto Yiangou Mark Price Jindrich Cinatl Wei-Feng Xue Martin Michaelis Anastasios D Tsaousis |
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| Institution: | 1. Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, UK;2. School of Biosciences, University of Kent, Canterbury, UK;3. Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, UK;4. School of Physical Sciences, University of Kent, Canterbury, UK;5. Institut für Medizinische Virologie, Klinikum der Goethe-Universität, Frankfurt am Main, Germany |
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| Abstract: | Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs. |
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| Keywords: | Cell culture COLO-680N Lipidomics Proteomics Atomic force microscopy Immunofluorescence microscopy Electron microscopy |
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