In vitro antifungal activity of the berberine and its synergism with fluconazole |
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Authors: | Renata Sayuri Iwazaki Eliana Harue Endo Tânia Ueda-Nakamura Celso Vataru Nakamura Lourdes Botelho Garcia Benedito Prado Dias Filho |
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Institution: | 1.Programa de Pós-gradua??o em Análises Clínicas,Universidade Estadual de Maringá,Maringá,Brazil;2.Departamento de Análises Clínicas,Universidade Estadual de Maringá,Maringá,Brazil |
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Abstract: | Berberine with and without fluconazole was tested by an agar disk diffusion assay in which clinical isolates of Candida albicans were applied onto yeast extract-peptone-dextrose agar plate. Berberine, which had no intrinsic antifungal activity at the
concentration tested, exerted a powerful antifungal activity in combination of fluzonazole. Combinations of berberine and
fluconazole were also tested by the checkerboard assay to determine whether they had favorable or unfavorable antifungal interactions.
The MIC of fluconazole was 1.9 μg/ml when the drug was tested alone and decreased to 0.48 μg/ml in the presence of berberine
concentrations of 1.9 μg/ml. However, berberine at concentrations of >1.9 μg/ml combined with a fluconazole supra-MIC (i.e.,
>1.9 μg/ml) eliminated the residual turbidity in the incubation wells. This endpoint fitted to the definition of MIC-0 (optically
clear wells) and reflected the absence of a trailing effect, which is the result of a residual growth at fluconazole concentrations
greater than the MIC. |
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