Micropropagation ofCereus peruvianus mill. (cactaceae) by areole activation |
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Authors: | Maria de Fátima P S Machado José Prioli |
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Institution: | (1) Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa, Parana, Brazil |
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Abstract: | Summary Currently,Cereus peruvianus plants can be rapidly clonedin vitro via adventitious organogenesis using callus cultures; however, somaclonal variation is a problem. A method is described herein
using lateral bud explants to produce multiple shoots for clonal propagation. Apical and lateral explants were cultured on
MS (Murashige and Skoog, 1962) media with factorial combinations of the auxins indole-3-acetic acid (IAA), 1-naphthaleneacetic
acid (NAA), and cytokinins 6-ben-zyladenine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (kinetin) at the concentrations
0.0, 0.01, 0.1, 1.0 mg“l−1. Positive results were obtained from the lateral explants in all conditions tested, but apical explants did not respond toin vitro multiplication ofC. peruvianus cactus at all growth regulator combinations tested. Formation of axillary shoots inC. peruvianus seems most frequent in medium containing BA at 1.0 mg·l−1 (4.44 μM) and IAA or NAA at 1.0 mg·l−1 (5.71 μM or 5.37 μM respectively), but the frequency of shoot formation in the BA or kinetin and NAA or IAA combinations indicated that any of
the combinations tested can be used for multiplication ofC. peruvianus plants regenerated from callus tissue culture. Root formation occurred in all (100%) of the cactus shoots after 9 wk in the
same culture medium. All the cacti that developed at the different auxin and cytokin combinations continued growth after transfer
to a potting mix of red earth (Paleudult) and ground river sand (1∶1). |
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Keywords: | Micropropagation cactus areole activation cactaceae tissue culture cerus peruvianus |
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