Use of RAPD in differentiation of selected species of <Emphasis Type="Italic">Sargassum</Emphasis> (Sargassaceae,Phaeophyta) |
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Authors: | C L Wong S M Ng S M Phang |
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Institution: | (1) Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Setapak Campus, Jalan Genting Kelang, Setapak, 53300 Kuala Lumpur, Malaysia;(2) Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia |
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Abstract: | Sargassum C. Agardh is one of the most common but little understood genera of Phaeophyta in Malaysia. The difficulty in species delineation
is due to morphological plasticity. A combination of morphology and random amplified polymorphic DNA (RAPD) studies of selected
Sargassum species was carried out to have a better understanding of the taxonomy. Primer OPA13 was found to be good for discriminating
between Sargassum species. Sargassum binderi was shown to be different from S. oligocystum (SD>0.5 = 14.11%), indicating the importance of the vesicle and receptacle in species differentiation. S. baccularia was clearly separated out from S. polycystum and S. stolonifolium using primer OPA13. RAPD analysis showed that the presence of the stolon is an important character for separating S. baccularia (no stolon) from S. polycystum (stolon) and S. stolonifolium (stolon).
Presented at the 6th Meeting of the Asian Pacific Society of Applied Phycology, Manila, Philippines. |
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Keywords: | Molecular taxonomy RAPD Sargassum |
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