Validity and applicability of membrane model systems for studying interactions of peripheral membrane proteins with lipids |
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Authors: | Aleksander Czogalla Michał Grzybek Walis JonesÜnal Coskun |
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Institution: | Laboratory of Membrane Biochemistry, Paul Langerhans Institute Dresden, Faculty of Medicine Carl Gustav Carus at the TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; German Center for Diabetes Research (DZD), Germany |
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Abstract: | The cell membrane serves, at the same time, both as a barrier that segregates as well as a functional layer that facilitates selective communication. It is characterized as much by the complexity of its components as by the myriad of signaling process that it supports. And, herein lays the problems in its study and understanding of its behavior — it has a complex and dynamic nature that is further entangled by the fact that many events are both temporal and transient in their nature. Model membrane systems that bypass cellular complexity and compositional diversity have tremendously accelerated our understanding of the mechanisms and biological consequences of lipid–lipid and protein–lipid interactions. Concurrently, in some cases, the validity and applicability of model membrane systems are tarnished by inherent methodical limitations as well as undefined quality criteria. In this review we introduce membrane model systems widely used to study protein–lipid interactions in the context of key parameters of the membrane that govern lipid availability for peripheral membrane proteins. This article is part of a Special Issue entitled Tools to study lipid functions. |
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Keywords: | AFM atomic force microscopy BLM black lipid membrane Cer ceramide CTX-B B subunit of cholera-toxin DPH diphenylhexatriene DTT dithiothreitol ER endoplasmic reticulum ESCRT endosomal sorting complexes required for transport FCS fluorescence correlation spectroscopy GM1 monosialotetrahexosylganglioside GPMV giant plasma membrane vesicle GUV giant unilamellar vesicle ITC isothermal titration calorimetry ITO indium tin oxide Ld liquid disordered Lo liquid ordered LUV large unilamellar vesicle MLV multilamellar vesicle NEM N-ethyl maleimide PA phosphatidic acid PC phosphatidylcholine PE phosphatidylethanolamine PEG polyethylene glycol PFA paraformaldehyde PI phosphatidylinositol PIP phosphatidylinositol phosphate PS phosphatidylserine PVDF polyvinylidene difluoride SLB supported lipid bilayer SM sphingomyelin SPR surface plasmon resonance SUV small unilamellar vesicle TIRF total internal reflection fluorescence TLC thin layer chromatography |
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