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Detection and molecular cloning of CYP74Q1 gene: Identification of Ranunculus acris leaf divinyl ether synthase
Authors:Svetlana S. Gorina  Yana Y. ToporkovaLucia S. Mukhtarova  Ivan R. ChechetkinBulat I. Khairutdinov  Yuri V. GogolevAlexander N. Grechkin
Affiliation:Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia
Abstract:
Keywords:DES, divinyl ether synthase   RaDES, Ranunculus acris divinyl ether synthase   HPL, hydroperoxide lyase   AOS, allene oxide synthase   13(S)-HPOT, (9Z,11E,13S,15Z)-13-hydroperoxyoctadecatrienoic acid   13(S)-HPOD, (9Z,11E,13S)-13-hydroperoxyoctadecadienoic acid   9(S)-HPOD, (9S,10E,12Z)-9-hydroperoxyoctadecadienoic acid   IMAC, immobilized metal affinity chromatography (IMAC)   TMS, trimethylsilyl   GC-MS, gas chromatography-mass spectrometry   ECL, equivalent chain lengths   ORF, open reading frame   IHCD, I-helix central domain, the catalytically important six amino acid domain in the centre of P450 I-helix
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