Solid-state NMR spectroscopy to study protein–lipid interactions |
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Authors: | Daniel Huster |
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Institution: | Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstr. 16-18, D-04107 Leipzig, Germany |
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Abstract: | The appropriate lipid environment is crucial for the proper function of membrane proteins. There is a tremendous variety of lipid molecules in the membrane and so far it is often unclear which component of the lipid matrix is essential for the function of a respective protein. Lipid molecules and proteins mutually influence each other; parameters such as acyl chain order, membrane thickness, membrane elasticity, permeability, lipid-domain and annulus formation are strongly modulated by proteins. More recent data also indicates that the influence of proteins goes beyond a single annulus of next-neighbor boundary lipids. Therefore, a mesoscopic approach to membrane lipid–protein interactions in terms of elastic membrane deformations has been developed. Solid-state NMR has greatly contributed to the understanding of lipid–protein interactions and the modern view of biological membranes. Methods that detect the influence of proteins on the membrane as well as direct lipid–protein interactions have been developed and are reviewed here. Examples for solid-state NMR studies on the interaction of Ras proteins, the antimicrobial peptide protegrin-1, the G protein-coupled receptor rhodopsin, and the K+ channel KcsA are discussed. This article is part of a Special Issue entitled Tools to study lipid functions. |
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Keywords: | AFM atomic force microscopy CP cross-polarization CSA chemical shift anisotropy DHA docosahexaenoic acid GPCR G protein-coupled receptor MAS magic-angle spinning NOE nuclear Overhauser effect NOESY nuclear Overhauser enhancement spectroscopy PC phosphatidylcholine PE phosphatidylethanolamine PG phosphatidylglycerol PFG pulsed field gradients ROS rod outer segment REDOR rotational-echo double resonance STD saturation transfer difference |
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