Structural organization of cholera toxin gene and its expression in an environmental non-pathogenic strain ofVibrio cholerae |
| |
Authors: | Uma Bhattacharyya Amit Ghosh Ranajit K. Ghosh |
| |
Affiliation: | (1) Indian Institute of Chemical Biology, Jadavpur, 700 032 Calcutta, India;(2) Present address: Department of Biochemistry, University of North Carolina, Chapel Hill, 27514 North Carolina, USA |
| |
Abstract: | Non-pathogenic, environmental strain ofVibrio cholerae, ELTOR Ogawa EW6 carries a copy of the cholera toxin gene in its chromosome. Restriction enzyme digestion followed by Southern blot analysis revealed that the structure of the cholera toxin gene in this organism is different from that found in the virulent strains. The xbaI site which has been found to be conserved in the cholera toxin of the virulent strains examined so far, is absent here. Results of the RNA dot blot analysis indicated that the cholera toxin gene in EW6 is transcribed much less efficiently compared to the cholera toxin gene present in the virulent strainVibrio cholerae classical Inaba 569B. |
| |
Keywords: | Vibrio cholerae avirulent strain toxin gene |
本文献已被 SpringerLink 等数据库收录! |
|