| 地中海拟无枝菌酸菌U32中amrC／amkC基因的序列分析及在大肠杆 … |
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| 引用本文： | 高瑾,赵国屏. 地中海拟无枝菌酸菌U32中amrC／amkC基因的序列分析及在大肠杆 …[J]. 生物工程学报, 2000, 16(6): 684-689 |
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| 作者姓名： | 高瑾 赵国屏 |
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| 作者单位： | [1]中国科学院上海植物生理研究所,上海 [2]中国科学院上海生物工程研究中心,上海 |
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| 基金项目： | 国家自然科学基金资助项目(39630010)。 |
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| 摘 要： | 从力复霉素ＳＶ产生菌－－地中海拟无枝菌酸菌（Ａｍｙｃｏｌａｔｏｐｓｉｓ ｍｅｄｉｔｅｒｒａｎｅｉ）Ｕ３２的硝酸盐同化基因簇的上游克隆了一个２．６ｋｂ的ＥｃｏＲＩ－ＸｈｏＩ ＤＮＡ片段并测定其序列。序列分析表明，该ＤＮＡ片段编码两个完整的开放阅读框架（ＯＲＦ），ＯＲＦ２的起始密码子ＧＴＧ与ＯＲＦ１的终止密码子ＴＧＡ在ＴＧ处重叠。ＯＲＦ１编码一个含２２４个氨基酸的多肽，它同放线菌中典型的应答调节蛋白包
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| 关 键 词： | 地中海拟无枝菌酸菌 序列分析 表达 力复霉素 |
| 文章编号： | 1000-3061(2000)06-0684-06 |
| 修稿时间： | 2000-02-10 |
Sequence Analysis of A Putative Two-component Signal Tranduction System mrC/amkC Gene from Amycolatopsis mediterranei U32 and Their Expression in Escherichia coli |
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| GAO Jin,WANG Wei-Wu,JIANG Wei-HongZHAO Guo-PingCHIAO Jui-Shen. Sequence Analysis of A Putative Two-component Signal Tranduction System mrC/amkC Gene from Amycolatopsis mediterranei U32 and Their Expression in Escherichia coli[J]. Chinese journal of biotechnology, 2000, 16(6): 684-689 |
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| Authors: | GAO Jin WANG Wei-Wu JIANG Wei-HongZHAO Guo-PingCHIAO Jui-Shen |
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| Affiliation: | GAO Jin,WANG Wei-Wu,JIANG Wei-Hong;(Laboratory of Molecular Regulation for Microbiology Secondary Metabolism, Shanghai Institute of Plant Physicology, Chinese Academy of Sciences, Shanghai 200032);ZHAO Guo-Ping;(Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233);CHIAO Jui-Shen;(Laboratory of Molecular Regulation for Microbiology Secondary Metabolism, Shanghai Institute of Plant Physicology, Chinese Academy of Sciences, Shanghai 200032) |
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| Abstract: | A 2.6kb Eco RI| Xho I fragment,from the upstream region of the nitrate assimilatory gene cluster of rifamycin SV_producing Amycoloatopsis mediterranei U32,was cloned and sequenced.Sequence analysis showed two complete ORFs.ORF1 and ORF2 are overlapped in the TGA of ORF1 and the GTG of ORF2.ORF1 encodes a polypeptide of 224 amino acids which displays high similarities with the typical response regulators of actinomycetes including AfsQ1 and MtrA,whereas ORF2 encodes a protein of 472 amino acids which displays homologies with the histidine kinase such as AfsQ2 and MtrB.The Two ORFs form aputative two|component signal tansduction system AmrC/AmkC.Fusion expression analysis in the T7 system showed that amrC encoded a fusion protein with an apparent molecular mass of 30kD,the trucated amkC encoded a polypeptide with molecular mass of 46kD. |
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| Keywords: | Amycolatopsis mediterrane two component signal transduction system response regulator histidine kinase sequence analysis |
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