Genetic bases of methionine dependence in <Emphasis Type="Italic">Yersinia pestis</Emphasis> strains of main and non-main subspecies |
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Authors: | G N Odinokov G A Eroshenko Ya M Krasnov L M Koukleva N Yu Shavina A I Pavlova V V Kutyrev |
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Institution: | 1.Mikrob Russian Anti-Plague Research Institute,Saratov,Russia |
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Abstract: | Structural and functional organization of genes responsible for biosynthesis of amino acid methionine, which plays a leading
role in cellular metabolism of bacteria, was studied in 24 natural Yersinia pestis strains of the major and non-main subspecies from various natural plague foci located in the territory of Russian Federation
and neighbouring foreign countries, and also in Y. pestis and Y. pseudotuberculosis strains recorded in the files of NCBI GenBank database. Conservatism of genes metA, metC, metE, and metH as well as regulatory genes metR and metJ involved in biosynthesis of this amino acid was established. Sequencing of the variable locus of gene metB in natural Y. pestis strains of major and non-main subspecies revealed that the reason for the methionine dependence of strains belonging to the
main subspecies is a deletion of a single nucleotide (−G) in the 988 position from the beginning of the gene, whereas this
dependence in strains belonging to subspecies hissarica results from the appearance of a single nucleotide (+G) insertion in the 989 position of gene metB. These mutations are absent in strains of the caucasica, altaica, and ulegeica subspecies of the plague agent and in strains of pseudotuberculosis microbe, which correlates with their capacity for methionine
biosynthesis. |
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