Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana |
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Authors: | Lee Mi Kyeong Lim Song Won Yang Hyekyung Sung Sang Hyun Lee Heum-Sook Park Mi Jung Kim Young Choong |
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Affiliation: | College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea. |
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Abstract: | Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4',7'-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4',7',4'-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4',7',4'-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure-activity relationship, methoxyl groups at 4' and 4' in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis. |
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