Metabolism of aromatic hydrocarbons by yeasts |
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Authors: | Carl E. Cerniglia Sidney A. Crow |
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Affiliation: | (1) Department of Microbiology, The University of Texas at Austin, 78712 Austin, Texas, USA;(2) Department of Biology, Georgia State University, 30303 Atlanta, Georgia, USA;(3) Present address: National Center for Toxicological Research, Food and Drug Administration, 72079 Jefferson, Arkansas, USA |
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Abstract: | Six yeasts were examined for their ability to metabolize naphthalene, biphenyl and benzo(a)pyrene. All of the organisms tested oxidized these aromatic hydrocarbons. Candida lipolytica oxidized naphthalene to 1-naphthol, 2-naphthol, 4-hydroxy-1-tetralone and trans-1,2-dihydroxy-1,2-dihydronaphthalene. The major metabolite was 1-naphthol. C. lipolytica oxidized biphenyl to produce 2-, 3-, and 4-hydroxybiphenyl, 4,4′-dihydroxybiphenyl and 3-methoxy-4-hydroxybiphenyl. 4-Hydroxybiphenyl was the predominant metabolite formed. C. lipolytica oxidized benzo(a)pyrene to 3-hydroxybenzo(a)pyrene and 9-hydroxybenzo(a)pyrene. Metabolites were isolated and identified by absorption spectrophotometry, mass spectrometry and thin-layer, gasliquid and high-pressure liquid chromatography. Where possible the structures of these metabolites were confirmed by comparison with authenic compounds. |
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Keywords: | Naphthalene Biphenyl Benzo(a)pyrene Biotransformation Yeasts Candida lipolytica |
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