Cytofluorometric determination of DNA base content in plant nuclei and chromosomes by the fluorochromes DAPI and Chromomycin A3 |
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Authors: | Ursula Leemann Fritz Ruch |
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Institution: | Department of General Botany, Swiss Federal Institute of Technology, CH-8092 Zürich, Switzerland |
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Abstract: | The fluorescence of DAPI (AT-dye) and Chromomycin A3 (GMA; GC-dye) was measured in mitoses and interphase nuclei of nine species of plants having moderate or strong fluorescent bands—or none at all. In Scilla sibirica chromosomes, band and non-band regions were analysed. The results are compatible with a linear base-dependent fluorescence of the two dyes; their fluorescence can thus be utilized for cytofluorometric base content determination. The measurement of fluorescence fading of DAPI gave identical curves in band and non-band regions, whereas a different fading pattern could be observed with another AT-dye (Hoechst 33258). CMA also yielded different fading curves in band and non-band regions, which indicates a structural difference of the chromatin-dye complex. |
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Keywords: | To whom offprint requests should be sent: Laboratory of Radiobiology and Environmental Health University of California San Francisco CA 94143 USA |
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