PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems |
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| Authors: | B A Andrews D M Head P Dunthorne J A Asenjo |
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| Institution: | (1) Biochemical Engineering Laboratory Department of Food Science and Technology, University of Reading, RG6 2AP Reading, Reading, England |
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| Abstract: | Summary PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity of protein separation in aqueous two-phase systems. |
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