Optimal control strategy for the enhanced production of cellulase enzyme using the new mutant Trichoderma reesei E-12 |
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Authors: | S K Rakshit V Sahai |
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Institution: | (1) Department of Chemical Engineering, Indian Institute of Technology, 600 036 Madras, India;(2) Biochemical Engineering Research Centre Indian Institute of Technology, 110 016 New Delhi, India |
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Abstract: | Cellulase enzyme production was enhanced using the mutant strain Trichoderma reesei, E-12, which was shown to be partially resistant to catabolite repression. An optimal profile for pH, which was found to be the critical environmental parameter, was determined using a rigorous mathematical optimization procedure. Semi-empirical models were used to minimize complications in the computation. A 30% increase in enzyme activity and productivity was obtained using the optimal pH strategy as compared to the pH cycling strategy.List of Symbols
a
1
, a
2
, a
3
d–1, d–2, d–3
coefficients of the polynomial in the generalized logistic growth model
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a
4, a
5, a
6 d–1, d–2, d–3
coefficients of the polynomial in the generalized logistic product model
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b
1 d–1
enzyme synthesis rate constant
-
b
2
d
–1
enzyme decay rate constant
-
b
3
power coefficient in the polynomial model for enzyme synthesis
-
H
Hamiltonian function
-
J
Objective function of the maximization procedure
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K
1 kg/m3
limiting cell mass concentration in biomass logistic model
-
K
s
kg/m3
saturation constant
-
K
s
kg/m3
saturation death rate constant
-
q
power coefficient in polynomial model
-
s kg/m3
substrate concentration
-
t d
fermentation time
-
T d
total fermentation time (=7 d)
-
x
10 kg/m3
initial biomass concentration
-
x
1 kg/m3
biomass concentration at time t
-
x
2 F.P.A
enzyme activity at time t
-
x
3 d
state variable replacing time term on the right hand side of biomass equation
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x
f kg/m3
final biomass concentration
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z
1, z
2, z
3
adjoint variable corresponding to state variable x
1, x
2, x
3
-
d–1
specific death rate
-
d–1
specific growth rate |
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Keywords: | |
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