首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Optimal control strategy for the enhanced production of cellulase enzyme using the new mutant Trichoderma reesei E-12
Authors:S K Rakshit  V Sahai
Institution:(1) Department of Chemical Engineering, Indian Institute of Technology, 600 036 Madras, India;(2) Biochemical Engineering Research Centre Indian Institute of Technology, 110 016 New Delhi, India
Abstract:Cellulase enzyme production was enhanced using the mutant strain Trichoderma reesei, E-12, which was shown to be partially resistant to catabolite repression. An optimal profile for pH, which was found to be the critical environmental parameter, was determined using a rigorous mathematical optimization procedure. Semi-empirical models were used to minimize complications in the computation. A 30% increase in enzyme activity and productivity was obtained using the optimal pH strategy as compared to the pH cycling strategy.List of Symbols a 1 , a 2 , a 3 d–1, d–2, d–3 coefficients of the polynomial in the generalized logistic growth model - a 4, a 5, a 6 d–1, d–2, d–3 coefficients of the polynomial in the generalized logistic product model - b 1 d–1 enzyme synthesis rate constant - b 2 d –1 enzyme decay rate constant - b 3 power coefficient in the polynomial model for enzyme synthesis - H Hamiltonian function - J Objective function of the maximization procedure - K 1 kg/m3 limiting cell mass concentration in biomass logistic model - K s kg/m3 saturation constant - K prime s kg/m3 saturation death rate constant - q power coefficient in polynomial model - s kg/m3 substrate concentration - t d fermentation time - T d total fermentation time (=7 d) - x 10 kg/m3 initial biomass concentration - x 1 kg/m3 biomass concentration at time t - x 2 F.P.A enzyme activity at time t - x 3 d state variable replacing time term on the right hand side of biomass equation - x f kg/m3 final biomass concentration - z 1, z 2, z 3 adjoint variable corresponding to state variable x 1, x 2, x 3 - delta d–1 specific death rate - mgr d–1 specific growth rate
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号