| 水稻miRNA3026启动子及硫氧还蛋白基因OsTxnDC9的克隆与分析 |
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| 引用本文： | 董娟,李佛生,罗枫雪,夏芳,朱淑华,唐琳.水稻miRNA3026启动子及硫氧还蛋白基因OsTxnDC9的克隆与分析[J].中国生物工程杂志,2016,36(1):29-37. |
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| 作者姓名： | 董娟 李佛生 罗枫雪 夏芳 朱淑华 唐琳 |
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| 作者单位： | 四川大学生命科学学院 成都 610064 |
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| 基金项目： | 国家自然科学基金(31070276和31270360)、四川省国际合作基金(2012HH0006)资助项目 |
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| 摘 要： | 硫氧还原蛋白基因 OsTxnDC9 是水稻miRNA3026的宿主基因。克隆出了水稻miRNA3026启动子,其总长度为1 477bp;构建出4个缺失片段,瞬时表达表明这个启动子为弱启动子。在此基础上克隆出水稻硫氧还原蛋白 OsTxnDC9 基因,其长度为480bp。生物信息学表明 OsTxnDC9 基因编码的氨基酸序列与二穗短柄草硫氧还原蛋白基因编码的氨基酸(XP_003573612.1)序列同源性最高。荧光定量PCR发现OsTxnDC9在水稻花粉一核中表达量最高,亚细胞定位表明其主要在细胞质中表达。这为探索miRNA3026启动子和宿主基因的关系奠定了基础。
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| 关 键 词： | 荧光定量 启动子 瞬时表达 硫氧还原蛋白 宿主基因 |
| 收稿时间： | 2015-10-19 |
Cloning and Expression Analysis of Rice miRNA3026 Promoter and Thioredoxin OsTxnDC9 |
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| DONG Juan,LI Fo-sheng,LUO Feng-Xue,XIA Fang,ZHU Shu-hua,TANG Lin.Cloning and Expression Analysis of Rice miRNA3026 Promoter and Thioredoxin OsTxnDC9[J].China Biotechnology,2016,36(1):29-37. |
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| Authors: | DONG Juan LI Fo-sheng LUO Feng-Xue XIA Fang ZHU Shu-hua TANG Lin |
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| Abstract: | The 1 477bp promoter of miRNA3026 was cloned, furthermore, four mutant fragments with different length of deletion were constructed and transient expression assay showed that the activity of miRNA3026 promoter is weak. OsTxnDC9 was cloned, the host gene of miRNA3026, which has an open reading frame of 482bp. Bioinformatic analysis indicated that OsTxnDC9 encodes a protein which is highest homology with Brachypodium distachyon. The expression of OsTxnDC9 was highest in one nuclear stage of pollen, which was examined by real-time polymerase chain reaction essay. Subcellular localization suggested that the protein was located in cytoplasm. This research laied the foundation for further exploring the relationship of miRNA3026 promoter and its host gene. |
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| Keywords: | Promoter Real-time polymerase chain reaction Thioredoxin Transient expression Host gene |
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