毕赤酵母工程菌高密度发酵研究与进展

毕赤酵母表达系统是近年来发展最为迅速的一种新型外源蛋白真核表达系统,被广泛应用于多种不同领域且成功表达了许多基因工程产品。高密度发酵技术已被广泛运用到毕赤酵母工程菌发酵工程当中。主要从毕赤酵母的表达常用菌株、载体及表型等方面介绍了其表达系统,从外源基因自身的特性、培养基的组成、温度、pH、溶氧量及补料流加策略方面阐述了对毕赤酵母高密度发酵的过程及蛋白质表达结果的影响,并对毕赤酵母工程菌高密度发酵进行了展望,为其今后的研究及应用提供借鉴。

Research Progress of High Density Fermentation Process of Pichia pastoris

Pichia pastoris expression system is a new foreign protein eukaryotic one which has been the rapid development of the technology in recent years, widely used in many different fields and many gene engineering products has been successfully expressed. The composition of Pichia pastoris expression system was introduced mainly from the aspects of the expression of common strain, vector and phenotype. It was elaborated in detail that how these effect the process of high cell density fermentation of Pichia pastoris and the result protein expression from the aspects of exogenous gene's own characteristics, the composition of culture medium and the tempreture, pH, dissolved oxygen and feeding strategy. At last, the Pichia pastoris high cell density fermentation was discussed and provides a reference for further research.