绿原酸对美国白蛾幼虫生长发育和解毒相关蛋白活性的影响

【目的】明确植物次生代谢物质绿原酸对美国白蛾Hyphantria cunea幼虫生长发育和解毒相关蛋白活性的影响。【方法】将含不同浓度绿原酸(0, 0.125%, 0.250%, 0.500%, 1.000%和2.000%)的人工饲料饲养美国白蛾幼虫，测定各组中5龄幼虫6 d内的死亡率, 各组中5龄幼虫在取食48 h时的营养效应指标，0.500%绿原酸处理组中幼虫(3龄幼虫开始)的生长发育，以及在取食36 h时各组中5龄幼虫肠道中的解毒相关蛋白活性。【结果】绿原酸处理组中美国白蛾5龄幼虫6 d内的死亡率随着人工饲料中绿原酸浓度的增大而逐渐升高，且显著高于对照组(取食含10% DMSO人工饲料的)。绿原酸对美国白蛾5龄幼虫的营养效应指标有显著影响，各处理组幼虫的相对取食量和近似消化率显著高于对照组，食物转化率显著低于对照组，除0.125%绿原酸处理组外，其余处理组幼虫的食物利用率和相对生长速率也显著低于对照组。0.500%绿原酸处理使美国白蛾3-5龄幼虫发育历期比对照组显著延长28.24%(3.7 d)，老熟幼虫(6-7龄幼虫)的发育历期缩短8.97%(0.7 d)，且幼虫总成活率、化蛹率、羽化率、雌雄性比及产卵量等各项指标均显著低于对照组。不同浓度绿原酸处理对美国白蛾幼虫肠道中的细胞色素P450酶(CYP450)、谷胱甘肽S-转移酶(GSTs)、尿苷二磷酸葡萄糖醛酸转移酶(UGT)和ABC转运蛋白(ABC transporters)的活性具有显著影响，但对羧酸酯酶(CarE)活性未见显著影响。【结论】绿原酸可影响美国白蛾幼虫的存活率、食物利用效率、生长发育和繁殖，而美国白蛾幼虫可能通过调节食物利用率和诱导其解毒相关蛋白活性从而对食物中绿原酸产生适应性。

Effects of chlorogenic acid on the growth and development and detoxification-relatedprotein activities inHyphantria cunea(Lepidoptera: Arctiidae) larvae

【Aim】 This study aims to unveil the effects of chlorogenic acid on the growthand development and detoxification-related protein activities in the larvae of the fallwebworm, Hyphantria cunea. 【Methods】 H. cunea larvae were fed with the artificial dietscontaining different concentrations of chlorogenic acid (0, 0.125%, 0.250%, 0.500%, 1.000%and 2.000%), and the mortality rates of the 5th instar larvae in various groups within 6 d,the nutritional efficiency parameters of the 5th instar larvae in various groups at 48 hafter feeding, the growth and development of the larvae (from the 3rd instar) in the 0.500%chlorogenic acid treated group, and the detoxification-related protein activities in thegut of the 5th instar larvae in various groups at 36 h after feeding were determined.【Results】 The mortality rates of the 5th instar larvae of H. cunea in the chlorogenicacid treated groups in 6 d increased with the increase of the concentration of chlorogenicacid in the artificial diets, and were significantly higher than that in the control group(fed on the diet with 10% DMSO). Chlorogenic acid had significant influences on thenutritional efficiency of the 5th instar larvae of H. cunea. The relative consumption rate(RCR) and approximate digestibility (AD) in the chlorogenic acid treated groups weresignificantly higher than those in the control group, while the efficiency of conversationof digested food (ECD) in the chlorogenic acid treated groups, and the efficiency ofconversation of ingested food (ECI) and relative growth rate (RGR) in the chlorogenic acidtreated groups except the 0.125% chlorogenic acid treated group were significantly lowerthan those in the control. The 0.500% chlorogenic acid extended the duration of the 3rd-5thinstar larvae by 28.24% (3.7 d) while shortened the duration of the mature larvae (6th-7thinstar) by 8.97% (0.7 d). Moreover, the developmental parameters such as the larvalsurvival rate, pupation rate, eclosion rate, female-to-male sex ratio and ovipositionamount in the 0.500% chlorogenic acid treated group were significantly lower than those inthe control group. Various concentrations of chlorogenic acid had significant influences onthe activities of CYP450, GSTs, UGT and ABC transporters in the gut of the 5th instarlarvae of H. cunea, but showed no significant effects on the CarE activity. 【Conclusion】Chlorogenic acid can affect the survival rate, the food utilization efficiency and thegrowth and development of H. cunea larvae. H. cunea larvae may adapt chlorogenic acid infood by regulating food utilization efficiency and inducing the activities of metabolicdetoxification-related proteins.